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Training Courses

MALDI Mass Spectrometry

(Courtesy of WITA GmbH & Dr. Irrgang, Max-Volmer Institut, TU-Berlin, Germany)

Following a short introduction into MALDI-MS theory this course rapidly shifts its focus toward the practical aspects: Tryptic digest of 2D-gels plugs, sample preparation, measurement, analysis of spectra, creating valuable mass lists from raw data, Mascot-Searching and critical assessment of protein ID-results. Hands-on experience can be gathered in crucial areas of sample preparation and MS-Maldi spectra analysis.

Day 1

9:00 - 9:45
Seminar: Introduction into MALDI mass spectrometry.

10:00 -12:30
Demonstration: Sample preparation of gel plugs.

13:30 -18:00
Supervised Training: MALDI target preparation.

Day 2

9:00 - 9:45
Seminar: Data evaluation of MALDI-MS fingerprint data

10:00 -12:30
Supervised Training: MALDI target preparation & measurement of samples.

13:30 -17:00
Supervised Training: Data evaluation of MALDI-MS spectra.

17:00 -18:00
Seminar: Discussion of results.