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Training Courses

ESI Mass Spectrometry


(Courtesy of Proteome Factory AG, Berlin, Germany)




After a short introduction into ESI-MS theory this course concerns the tryptic digest of 2D-gels plugs, sample preparation, measurement and data evaluation for nano-LC-ESI-mass spectrometry. Hands-on experience can be gathered in the crucial areas of sample preparation and ESI-MS spectra analysis.


Day 1

9:00 - 9:45
Seminar: Introduction into nano-LC ESI mass spectrometry.

10:00 -12:30

Demonstration: Sample preparation of gel plugs.

13:30 -18:00

Supervised Training: Measurement of prepared samples.


Day 2

9:00 - 9:45
Seminar: Data evaluation of ESI-MS/MS data

10:00 -12:30

Supervised Training: Measurement of samples from gel plugs.

13:30 -17:00

Supervised Training: Data evaluation of spectra.

17:00 -18:00

Seminar: Discussion of results.