Training Courses
2D-NEPHGE
(Courtesy of Proteome Factory AG, Berlin, Germany)
This course focuses to train your practical skills in high-resolution 2D-NEPHGE technique. Special emphasis will be laid onto the manufacturing and evaluation of large (20x30cm) and giant format (40x30cm) gels.
Subsequent to the demonstration of each technique by skilled personel, each participant has the opportunity to prepare own large format 2D-NEPHGE gels. Hands-on experience can be obtained for crucial procedures like sample preparation, gel handling and data evaluation.
Day 1
9:00 - 9:45
Seminar: Introduction into 2D-gel techniques
10:00 - 12:30
Demonstration: Purging of 1st dimension large and giant tube gels.
Supervised Training: Purging of 1st dimension large tube gels.
13:30 - 18:00
Demonstration: Termination of 1st dimension large tube gels, application onto 2nd dimension.
Supervised Training: Start of over night run.
Day 2
9:00 - 9:45
Seminar: Practical aspects of 2D-gel technique.
10:00 - 12:30
Demonstration: Termination of 2nd dimension large gels.
Supervised Training: Sample application onto 1st dimension tube gels and start of run.
13:30 - 18:00
Supervised Training: Staining of 2nd dimension large gels. Termination of 1st dimension large gels. Application of tube gels onto 2nd dimension. Start of over night run.
Day 3
9:00 -11:00
Demonstration: Scanning of gels.
11:00 -12:30
Seminar: Evaluation of 2D-gels.
13:30 -18:00
Seminar: Evaluation of 2D-gels (continued).
Supervised Training: Staining 2nd dimension large gels.
Supervised Training: Application of samples onto 1st dimension giant gels. Start of over night run.
Day 4
9:00 -11:00
Supervised Training: Termination of 1st dimension giant gels, application onto 2nd dimension. Start of run.
11:00 -12:30
Supervised Training: Evaluation of 2D-gels.
13:30 -18:00
Supervised Training: Evaluation of 2D-gels (continued).
Supervised Training: Termination of 2nd dimension large gels. Start of staining.
Day 5
9:00 -12:30
Supervised Training: Scanning of all large gels prepared. Computer assisted 2D-gel evaluation.
13:30 -17:00
Supervised Training: Evaluation of 2D-gels (continued).
17:00 -18:00
Seminar: Discussion of results.
